Welcome to AABB's Bringing Flow Cytometry to Life: Applications in Cellular Therapies course.
Flow cytometry plays an integral role in the modern laboratory. This rapid, high throughput, multiparameter assay is used to phenotype, characterize, and assess cell function by measuring marker expression on the cell surface and inside cells. It is an important tool that helps to ensure quality, purity, safety and/or potency of products used in cellular therapies and regenerative medicine. Learning how flow cytometry is applied in practice is essential to maximizing its power to collect and interpret data for clinical and research applications.
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Instructions & Additional Information
Understanding of flow cytometry technology, function and processes involved in sample preparation and measurement is important to ensuring consistent and reliable results. This section describes the technology and the ways in which flow cytometry can be optimized for CT products.
Following completion of this section, learners should be able to:
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Section 1 Foundational Concepts in Flow Cytometry
There are a variety of methods to apply flow cytometry to a range of different CT products. This section presents information on application of current flow cytometric methods to a variety of cell types serving both as current practices information as well as an introduction to methods that may prove useful in thinking about approaches to other flow strategies for future products. This section presents examples of how flow cytometry is used in a variety of applications.
Following completion of this section, learners should be able to:
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Section 2: Cellular Therapy Applications of Flow Cytometry
Standardization of flow cytometry testing is very important for delivery of consistent quality CT products particularly with the increase in multi-center trials. With development of some cell types there may not be a “one size fits all” approach, but there are general points to consider in standardization. This section pulls the concepts together testing your knowledge and things to consider in a series of exercises providing feedback.
Following completion of this section, learners should be able to:
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Section 3: Review of Concepts: Applying What You Have Learned
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Self-Assessment
In an ongoing effort to provide the highest quality education, we would like to get your feedback. Completion of this evaluation is required to access your continuing education credit certificate.
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Evaluation
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