As life-saving as it is, the basic centrifugal technology behind current apheresis and cell processing devices has remained since their invention. In this session, Dr. Thibodeaux will discuss her team’s method to optimize density centrifugation technology to improve the efficiency of red cell exchange, thus providing a theoretically improved alternative to isovolemic hemodilution to decrease red cell unit usage or increase time intervals between exchanges. Dr. Manis/Fiering will discuss his team’s work using acoustic separation technology to 1) purify lymphocytes and 2) optimize sickle cell gene therapy by selectively enriching for hematopoietic stem cells and improve poor collection efficiency observed with stem cell collections in patients with sickle cell disease owing to the altered centrifugation patterns of sickle red cells. Dr. Chalmers will discuss his team’s work using continuous flow magnetic cell sorting to 1) separate red cells, including sickle from normal donor, based on their intrinsic paramagnetic properties and 2) distinguish relevant cell subsets (such as high-expressing CD34+ cells for gene therapy) by level of antigen expression using magnetically labeled antibodies.
Appraise the science behind developing technologies in continuous cell separation other than density or counterflow centrifugation, such as acoustic and magnetic separation technologies
Compare standard density centrifugation methods with newer separation technologies to selectively remove recipient (sickle) and not donor red cells
Describe how newer methods can selectively enrich for lymphocytes, hematopoietic stem cells or subsets, and/or enrich or deplete red cell populations.
**This education session will not offer CME. You will be able to claim CE for the session.
Please note: Jason Fiering did not consent to be recorded; therefore, the on-demand presentation and handout have been modified accordingly.