Error loading player: No playable sources found

AM23-MN-05-O

AM23-MN-05-O: Blood Group Genotyping and Interpretation of Variant RBC Antigens Using Bioinformatic Tools (Enduring)

Credits
1 General Continuing Education (GEN) | 1 Florida Lab Personnel (FLP) | 1 California Nurse (CN) | 1 California Lab Personnel (CLP) | 1 Physician (PHY)
$30
Standard Price
Members save $5

In the past few decades, blood group genotyping has greatly expanded our knowledge of the more prevalent alleles, paving the way for commercial genotyping assays. These assays have improved efficiency in providing genomic results, but understanding assay limitations can help direct additional investigations for complex cases.Variant alleles may affect protein expression and can be associated with alloimmunization risk after exposure through transfusion, pregnancy, or transplant. While genomic testing has increased understanding of altered antigens, alloimmunization risk remains unknown for many antigens encoded by variant alleles. Bioinformatic tools, including 3D protein modeling, can help assess the impact of amino acid changes encoded by variant alleles and provide insight into alloimmunization risk. When a variant allele is identified, blood bankers and clinicians must understand its clinical impact. In this program, we will review variant alleles, how we detect them, and the difficulty of predicting the risk of alloimmunization. We’ll propose using bioinformatics to gain insight into antigen expression in these cases.

Learning Objectives

  • Discuss common genetic mechanisms that lead to variant (or altered) alleles.
  • Describe genotyping methods used to detect and identify variant alleles, including limitations.
  • Explain the challenges of predicting alloimmunization risk and clinical significance of antibodies associated with variant antigens.
  • Discuss the use of in silico tools and 3D protein modeling to evaluate the effect of amino acid changes and provide insight into alloimmunization risk.

Moderator

Speaker Image for Lynsi Rahorst
Lynsi Rahorst, MHPE, MLS(ASCP)SBB
New York Blood Center Enterprises

Speakers

Speaker Image for Aline Floch
Aline Floch, MD, PhD
Associate professor, Etablissement francais du sang, Hopital Henri Mondor, Univ. Paris Est Creteil, INSERM U955, IMRB, Creteil, France
Speaker Image for Sunitha Vege
New York Blood Center

Tracks

Related Products

Thumbnail for AM24-MN-28-O: Choose Your Own Adventure: Exploring the Depths of Serology and Peaks of Genomics (Enduring)
AM24-MN-28-O: Choose Your Own Adventure: Exploring the Depths of Serology and Peaks of Genomics (Enduring)
What do you do when you discover an unexpected antigen typing? Do you investigate history, rule out sample switch, opt for a different serologic technique, or refer for DNA testing? What about when you encounter an antibody of unidentified specificity? Which test do you perform next, and once the a…
Thumbnail for AM23-ST-06-O: Deciphering the Clinical Clues: Updates to Protocols and Procedures for Anti-CD47 Agents in Clinical Laboratories (Enduring)
AM23-ST-06-O: Deciphering the Clinical Clues: Updates to Protocols and Procedures for Anti-CD47 Agents in Clinical Laboratories (Enduring)
_This program has been supported by an independent educational grant from Gilead Sciences, Inc…
Thumbnail for AM23-ST-08-O: Better Together: Serology and Genomics to Resolve Red Blood Cell and Platelet Antibody Cases (Enduring)
AM23-ST-08-O: Better Together: Serology and Genomics to Resolve Red Blood Cell and Platelet Antibody Cases (Enduring)
Detection and identification of antibodies is critical to providing the safest and most effective red blood cell and platelet transfusions…